Experimental design and statistical analyses for validation of OpenCASA For validation of the OpenCASA chemotaxis module, we simulated a high number of sperm populations using the persistent random walk model described above.
Fig 7. Verification of the ch-index in a non-chemotaxis condition. Validation of motility module Sperm motility was first evaluated by a commercial computer-assisted sperm analyzer ISAS, Version 1. Table 4. Table 5. Table 6. Supporting information. S1 Fig. Bland-Altman test for motility parameters. Results obtained for motility in the validation test.
S2 Fig. Bland-Altman test for morphometry parameters. Results obtained for morphometry in the validation test. S3 Fig. Bland-Altman test for viability parameters. Results obtained for viability in the validation test. S1 File. Software Design Document.
References 1. Mortimer ST. J Androl. Amann RP, Waberski D. J Assist Reprod Genet. View Article Google Scholar 4. Sperm morphology assessment: historical review in relation to fertility. Hum Reprod Update. Automated sperm head morphology analyzer for open-source software. The relationship between ram sperm head morphometry and fertility depends on the procedures of acquisition and analysis used.
Biol Reprod. Harrison R a. P, Vickers SE. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil. Bahat A, Eisenbach M. Microscope with phase contrast to visualize the sample. See hardware requirements. See editions comparison. Maintenance Annual maintenance service for software updates and technical support.
Customized reports Custom reporting service that offers the ability to customize the report template.
Documents and support. Additional information. It also provides dilution information to extend the entire ejaculate or just a single dose. Reproductive toxicology testing for a variety of substances, such as pharmaceuticals and chemicals. A majority of male reproductive toxicology studies are conducted using the rat model.
In addition, reproductive toxicology testing is commonly performed on fish sperm, to test for adverse effects of environmental contaminants. Our CEROS II spern analyer is most popular for fish studies and an option for a cooled stage increases compatibility with cold water fish sperm. Reproductive toxicology laboratories assess the effect of chemicals and pharmaceuticals on reproductive function. In most instances, the initial testing uses rats as the animal model. Becoming our distributor, you take advantage of being system integrator: sell suitable microscopes, counting chambers , replaceable items, dyes and lots of other complimentary equipment to provide complete customer support.
We are always ready to share information where and what to buy at the best prices. We are ready to provide any consultancy on how to integrate a complete CASA and provide extensive customer support including installation and training. Since we are a team of professional image analysis software developers, we never stand still and continue to improve the software.
Software development is never finished and you can count on adding special features required by your customers. Our database is specially tailored for image analysis needs. You can catalogue virtually any digital assets corresponding with sperm analysis in one place and have fast and smart access to any files. The volume of semen analysis data , images, clips and documents in your database is restricted only by the size of your storage media.
Your sperm analysis database can be stored on server in your local network which will enable all the security policies and backup procedures provided by Windows. Internal archiving tool will help you to keep your important semen analysis and patient data save as well as to make archives with older information to free your storage space. There are many security tools and procedures to ensure the integrity of your sperm analyzer data.
Database records are deleted to internal recycle bin and removed permanently only after you empty your bin. Images and videos are deleted to Windows recycle bin and can be restored. To prevent unwanted changes in the database records, all the records are locked for editing until you press a special button. To avoid unwanted changes by unauthorized personnel, you can enable PIN mode. Database structure is implemented as a tree of folders with separate technical files, images, videos, 3rd-party files for every record.
This provides the advantage of loading only one record at a time, which makes your work with a large database fast and effective. Furthermore, storage of data in multiple files will protect you in case your PC is infected with a virus: if the virus destroys some files, you will lose only several records and in no case the whole database.
The database is written in XML Unicode which provides wide options of integration of your data in 3rd-party databases as well as the ability to use your local language to fill in the database fields.
Database structure can be easily adjusted by the user through a simple and visually clear interface. Add fields for storing text data also multiline fields for large amounts of text, drop-down lists with predefined values or automatically generated lists for fast selection of repeating words and phrases , numerical data e. Move single fields or large groups of fields to organize your working space.
Rename fields as you need, add any number of fields to store additional sperm analysis data along with automatically calculated parameters. Use fast search option to find required data in the fields or to retrieve images, video, documents.
Apply extended filters to make selection of records according to any number of parameters e. Easy filters can be created fast, complicated multiparametric filters can be saved to filter list and reapplied any time.
Numeric fields can be set up to count records in the database automatically according to required parameters e. Special autotext box allows you to make an extended tree of predefined text blocks nomenclature, ready-made phrases describing the diagnosis, prescriptions etc. Making an advanced tree will let you fill in the patient record with a few clicks without the need of typing long phrases again and again which saves your time and helps to avoid misprints in the record. Automatic diagnosis.
Autotext box also contains special tool which combines database filters and autoinsertion of text blocks to required text fields. Create a filter describing a diagnosis if a number of parameters meets normal limits and select this filter as condition of insertion of the diagnosis into corresponding field. This will allow you to provide exact automatic diagnosis with one click without the need to look through a number of parameters and compare them to normal values.
Multilanguage support. If your clinic provides consulting in several languages, using autotext insertion based on filters, you can add diagnosis and other text blocks in as many languages as you need. Add a field marking required language and translate your text with one button. Database can be displayed in the table mode which allows you to keep track of all the statistics and make your own local reference values of all the measured parameters.
The analysis is based on frame by frame detection of sperm heads on video clips and building precise tracks which reveal the nature of sperm movement and provide sperm concentration value.
The total number of sperms is also calculated automatically. One frame with tracks is automatically saved to database record to serve as visual support in the report or you can save a suitable frame yourself. Time-average velocity of a sperm head along its actual curvilinear path, as perceived in two dimensions in the microscope. Time-average velocity of a sperm head along the straight line between its first detected position and its last.
Certified for Windows Vista The "Certified for Windows Vista" logo is a compatibility designation for applications and devices that have passed a rigorous testing program on computers that are running Windows Vista. Regardless of software, CASA evaluation of sperm. The AutoSperm system has been discontinued. These pages are for documentation only. Permission to incorporate this software into commercial products may be obtained by contacting the Office of Technology Management at the University of California San Francisco [Sunita Rajdev, Ph.